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Preparation
- Wash Hands
Thoroughly wash your hands with soap and water or use hand sanitizer to ensure cleanliness. - Assemble Materials
You will need:- Lyophilized peptide vial
- Sterile vial of bacteriostatic water or other reconstitution solution
- Two sterile needles and syringes (one for reconstitution/extraction, one for injection)
- Alcohol swabs
- Sharps container (for safe disposal of needles and syringes)
- Sanitize Work Area
Clean your workspace with an alcohol wipe to reduce contamination risks. - Inspect Vials
Ensure the vials are sealed, sterile, and within the expiration date. Check for any damage or contamination.
Step 1: Reconstitution (Mixing the Peptide)
- Sanitize Vial Tops
Use an alcohol swab to clean the rubber stopper on both the peptide vial and the bacteriostatic water vial. - Attach First Needle
Attach a sterile needle to a syringe for reconstitution. - Draw Bacteriostatic Water
Insert the needle into the water vial, draw the desired volume (as indicated by your instructions or prescribed dosage), and remove any air bubbles by tapping the syringe and pushing the plunger slightly. - Inject Water into Peptide Vial
Insert the needle into the peptide vial at an angle to prevent foam or bubbles. Slowly inject the bacteriostatic water along the inner wall of the vial. - Dissolve Peptide
Gently swirl the vial (do not shake) until the peptide is fully dissolved. Avoid agitation as it may degrade the peptide.
Step 2: Extracting the Peptide
- Attach Second Syringe to Fresh Needle
Use a new sterile syringe and needle to maintain sterility. - Sanitize Peptide Vial Top
Clean the rubber stopper of the peptide vial again with an alcohol swab. - Draw Peptide Solution
Insert the needle into the vial and draw the desired dose into the syringe. Remove air bubbles by tapping and slightly pressing the plunger.
Step 3: Injection
- Prepare Injection Site
Select the injection site (commonly subcutaneous for peptides, such as the abdomen). Clean the area with an alcohol swab in a circular motion and let it dry. - Replace Needle (Optional)
If desired, switch to a smaller needle for injection (e.g., an insulin needle) for comfort. - Insert Needle
Pinch the skin at the injection site to create a fold. Insert the needle at a 45° to 90° angle, depending on the type of injection. - Inject the Solution
Slowly push the plunger to inject the peptide solution. - Withdraw Needle
Carefully remove the needle and immediately discard it into a sharps container. - Apply Pressure
Apply gentle pressure to the injection site with a clean cotton ball or tissue if needed.
Post-Injection Care
- Monitor for Reactions
Observe the injection site for redness, swelling, or other signs of irritation. Contact a healthcare provider if you notice any unusual reactions. - Dispose of Materials Safely
Place all used needles and syringes in the sharps container. - Store Remaining Peptides
If you have leftover peptide solution, store it in the refrigerator as per the instructions on the vial. Avoid freezing.
Important Notes
- Always follow your healthcare provider’s instructions for dosage and administration.
- Ensure sterility at every step to minimize the risk of infection.
- If you are unsure about any part of the process, consult a healthcare professional for guidance.
Step 1: Reconstitution (Mixing Peptide)
- Needle Size:
- Gauge: 18G–22G (wide enough for easy drawing of bacteriostatic water).
- Length: 1 inch (sufficient to penetrate the rubber stopper of vials).
This needle is used only to draw and inject the reconstitution liquid into the peptide vial. A wider gauge reduces resistance and minimizes wear on the rubber stoppers.
Step 2: Extraction (Drawing Peptide Solution)
- Needle Size:
- Gauge: 18G–22G (same as above).
- Length: 1 inch.
This size is used to draw the reconstituted peptide solution from the vial. Switching to a fresh needle for injection ensures sterility and prevents dullness.
Step 3: Injection (Subcutaneous Injection)
- Needle Size:
- Gauge: 27G–31G (thin for minimal discomfort).
- Length: 3/8 inch to 1/2 inch (suitable for subcutaneous injections, which only need to penetrate the fatty layer beneath the skin).
A short, fine needle such as an insulin syringe is often used for peptide injections for comfort and accuracy.
Key Considerations:
- Sterility: Always use a fresh, sterile needle for each step to avoid contamination.
- Injection Type: If the injection is intramuscular instead of subcutaneous, use a longer needle:
- Gauge: 22G–25G.
- Length: 1–1.5 inches.