AOD 9604 + HA

• Molecular Formula:C78H123N23O23S2
• Molar Weight: 1815.1 g/mol
• Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-lle-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-LysThr-Glu-Thr-Gin-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thy-lleGlu-Gin-Glu-Lys-Gin-Ala-Gly-Glu-Ser

DESCRIPTION

AOD9604 is a GH fragment which comprised the last 16 amino acids of the larger growth hormone molecule. Although originally studied for fat loss, further studies have transitioned it’s application for regenerative medicine. In combination with hyaluronic acid (HA), it is now being used to help regenerate hyaline cartilage and is showing strong efficacy in the treatment of osteoarthritis. The combination acts to enhance the differentiation of adipose mesenchymal stem cells into bone, promote proteoglycan and collagen production in chondrocytes, and promote differentiation of myoblasts into C2C12 cells; all of which are essential for bone, cartilage, and muscle repair.

These studies indicate that it has stronger therapeutic benefits compared to Bone Marrow Aspirate Concentrate (BMAC) and Platelet Rich Plasma (PRP) therapy, which have also been emerging as candidates for osteoarthritis medications. AOD9604 + HA has proceeded to human WOMAC trials which allow the combination to be investigated for on an osteoarthritis index which considers pain, stiffness, and functionality on a variety of scores.

PROTOCOL

• Content & Potency: AOD 1000mcg/ml + HA 10mg/ml intra-articular injectableprovided in a 5ml vial.
• Suggested dosage: 0.5-0.75m injected intra-articularly by a medical professionalonce a week for 4 weeks, then once a month for 5 months.

CLINICAL RESEARCH

Ann Clin Lab Sci. 2015 Summer;45(4):426-32.

Effect of Intra-articular Injection of AOD9604 with or without Hyaluronic Acid inRabbit Osteoarthritis Model.

Kwon DR1, Park GY2.

Objective: To investigate the effects of AOD9604 intra- articular injections with or without hyaluronic acid (HA) in a collagenase-induced knee osteoarthritis (OA) rabbit model.

Design: Mature New Zealand white rabbits (n=32) were randomly administered 2 mg collagenase type II twice in each knee joint. Weekly injections of 0.6 mL saline (Group 1), 6 mg HA (Group 2), 0.25 mg AOD9604 (Group 3), and 0.25 mg AOD9604 with 6 mg HA (Group 4) were administered for 4-7 weeks after the first intra-articular collagenase injection. The degree of cartilage degeneration was assessed using morphological and histopathological findings, and the degree of lameness was observed at 8 weeks after the first collagenase injection.

Results: Mean gross morphological and histopathological scores were significantly higher in Group 1 than in Groups 2, 3, and 4, and the scores were significantly lower in Group 4 than in Groups 2 and 3. The lameness period in Group 4 was significantly shorter than those in Groups 1, 2, and 3. The lameness period in Group 1 was significantly longer than those in Groups 2 and 3.

Conclusion: Intra-articular AOD9604 injections using ultrasound guidance enhanced cartilage regeneration, and combined AOD9604 and HA injections were more effective than HA or AOD9604 injections alone in the collagenase-induced knee OA rabbit model.