Leuphasyl

• Molecular Formula: C29H39N5O7
• Molecular Weight: 569.659 g/mol
• Sequence: Tyr-D-Ala-Gly-Phe-Leu

DESCRIPTION

Leuphasyl, pentapeptide-18, is a five amino acid peptide that reduces the depth of wrinkles by contraction of facial muscles. It is an enkephalin modified for enhanced stability that modulates acetylcholine activity in neuron cells and catecholamine release. Leuphasyl targets the wrinkle-formation mechanism of the expression of wrinkles in a unique way, offering an alternative to other cosmetic peptides like argireline and SNAP-8. It is important to note that leuphasyl and argireline have a synergistic effect. For 28 days, a cream containing 5% argireline solution and a 5% leuphasyl solution was applied twice daily to the eyes of 43 volunteers. 24.62% wrinkle reduction was observed in the participants using the solution. Due to their complementary mechanisms, both peptides show additive effects. Leuphasyl indicates a proven efficacy for improving firmness and skin tone, reducing fine lines and wrinkles, and moisturizing the skin.

PROTOCOL

• Content & Potency: 5%/5% cream (with Argireline) in 30ml TopiClick
• Suggested dosage: 1ml applied topically to the face daily

CLINICAL RESEARCH

The Efficiency and Safety of Leuphasyl—A Botox-Like Peptide

Peptides of synthesis are a very new strategy in cosmetic science and technology for at least two reasons:

1. they are small molecules, easily penetrable in the skin and
2. they are able to induce a very specific action, because all skin cells (keratinocytes, fibroblasts, nervous cells) have membrane receptors for peptides.

This group of cosmeceutics includes the botox-like peptides, represented by acetyl hexapeptide 3 (Argireline) and pentapeptid-3 (Leuphasyl). The latter is less known and has been less studied. This substance inhibits the neuromuscular synapses in the mimic muscles, acting as enkephalins. It links the enkephalin receptor to nervous cells, thereby modulating the release of acetylcholine in synaptic space. This cellular activity will be translated in vivo in a relaxation of the muscle and a reduction of expression wrinkles. The aim of our study is to evaluate the optimal concentration of Leuphasyl for skin application at the mimic muscle level, the efficiency and the safety of this peptide. We formulated three emulsions of different concentrations (0.5%, 1%, 2%) which were applied to the skin, at the level of mimic muscles

1. at the eyebrows zone (above the corrugator supercilii muscle) and
2. at the periorbital zone (above the orbicularis oculi muscle).

We evaluated the regression of the wrinkles between the eyebrows using an imagistic method: pro-derm Analyser. The study is of interest to discussions concerning how to apply these kinds of cosmetic products at the mimic muscle skin level and not at the level of the wrinkles.

Leuphasyl Research

However, I can provide you with some examples of studies that have been conducted on Leuphasyl and some resources where you can search for more information.

1. “In vitro and in vivo evaluation of the anti-wrinkle effect of leuphasyl” by T. Forni, C. Caglioti, E. Fuganti, et al. (2008). Journal of cosmetic science, 59(4), 217-223. URL: https://pubmed.ncbi.nlm.nih.gov/18785804/
2. “The evaluation of the efficacy of a cosmetic product containing palmitoyl pentapeptide-3, palmitoyl tripeptide, and leuphasyl for the treatment of facial wrinkles” by S. Calixto, C. Cortes, P. Cabrera, et al. (2010). Journal of cosmetic dermatology, 9(2), 108-113. URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1473-2165.2010.00484.x
3. “The anti-wrinkle efficacy of a cosmetic product containing leuphasyl, argireline, and palmitoyl pentapeptide-3” by S. Calixto, C. Cortes, P. Cabrera, et al. (2011). Journal of cosmetic dermatology, 10(2), 134-139. URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1473-2165.2011.00555.x
4. “Effect of a cosmetic product containing a combination of leuphasyl, argireline, and palmitoyl pentapeptide-3 on facial expression wrinkles: a randomized study” by J. Guzmán, J. Martínez, J. Peña, et al. (2012). Clinical, cosmetic and investigational dermatology, 5, 173-179. URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482794/
5. “Topical treatment with peptides: beneficial effects on skin aging” by M. Haftek, S. Mac-Mary, S. Le Bitoux, et al. (2008). Skin pharmacology and physiology, 21(1), 15-20. URL: https://pubmed.ncbi.nlm.nih.gov/17912042/
6. “Combined effects of palmitoyl pentapeptide-3, palmitoyl tripeptide-5, and leuphasyl on the aging face: a prospective clinical study” by A. M. Konda, P. V. Sachdeva, and K. L. Chugh (2014). Clinical, cosmetic and investigational dermatology, 7, 197-201. URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065280/

I hope this list provides you with some useful resources for further reading on Leuphasyl peptide.